硝酸盐、亚硝酸盐一直被认为是引起消化道恶性肿瘤的化学致癌物前体之一^[1-5]。饮食中98%硝酸盐食入后在胃和十二指肠吸收入血, 血液中的硝酸盐约25％被唾液腺摄取分泌到唾液进入口腔中^[6]。以往的研究表明腮腺是分泌硝酸盐的主要腺体之一上海陪同口译, 腮腺破坏后将导致口腔中硝酸盐及亚硝酸盐含量明显减少^[7]。体外抑菌实验表明, 亚硝酸盐具有抑制口腔主要致病菌生长的作用^[8]。涎腺功能低下能够导致唾液中硝酸盐、亚硝酸盐减少, 同时造成唾液细菌数量的增加^[9]。补充高浓度硝酸盐是否有预防涎腺功能低下者口腔细菌过度生长作用? 安全性如何等未见报道。本研究在小型猪腮腺萎缩模型上, 通过长期给予硝酸盐负荷, 探讨了通过高浓度硝酸盐饮食来补充唾液硝酸盐的可能性及其对口腔黏膜组织结构等远期作用影音翻译。

Nitriate and nitrite have been long recogonized as one of the chemical precarcinogen causing malignant tumor of digestive tract^[1-5]. 98%of the nitrate in diet was absorbed in stomach and duodenum into blood; 25% of the nitrate in blood was taken by salivary gland to secrete into saliva and finally into oral cavity^[6]. Previous study demonstrated that parotid gland was one of the main glands that secretes nitrite^[7]. In vitro bacteriostasis experiment showed that nitrite could effectively inhibit main pathogenic bacteria growth^[8]. Hypofunction of salivary gland reduced nitrate and nitrite in saliva^[9]. There’s no report about whether supplement of high concentration of nitrate can prevent bacterium overgrowth in people with hypofunction of salivary gland, and its safety. Based on the parotid atrophy minipig models, the present study investigates the possibility of supplimenting saliva nitrate by administrating high concentration nitrate diet and its long-term effect on oral cavity mucosa histological structure.